Journal: eNeuro

Article Title: Lack of Oncomodulin Increases ATP-Dependent Calcium Signaling and Susceptibility to Noise in Adult Mice

doi: 10.1523/ENEURO.0142-26.2026

Figure Lengend Snippet: Schematic representing altered ABR waveforms and Ca 2+ transients in Ocm −/− and Ocm +/+ OHCs with moderate (95 dB SPL) noise exposure. In Ocm +/+ mice, moderate noise induces slight latency and amplitude shifts, increased purinergic receptor expression, and increased Ca 2+ signaling. Ocm −/− OHCs exhibit elevated Ca 2+ signaling prenoise exposure and exhibit more dramatic latency and amplitude shifts postnoise exposure with no significant change in purinergic receptor expression after moderate noise exposure.

Article Snippet: Samples were labeled with antibodies to myosin 7A (Proteus Biosciences, number 25-6790, 1:200), the C-terminal-binding protein 2 (CtBP2, BD Biosciences #612044, 1:200), OCM (Santa Cruz Biotechnology, sc-7446, 1:200), GluR2 (Millipore, MAB397, 1:200), and P2X2 (Alomone Labs, APR-003, 1:400).

Techniques: Expressing

Journal: eNeuro

Article Title: Lack of Oncomodulin Increases ATP-Dependent Calcium Signaling and Susceptibility to Noise in Adult Mice

doi: 10.1523/ENEURO.0142-26.2026

Figure Lengend Snippet: P2X2 expression was upregulated in Ocm +/+ but not Ocm −/− mice after noise. A , B , Maximum projections of P2X2 immunolabeling in OHCs harvested from pre- and postnoise Ocm +/+ and Ocm −/− mice. P2X2 (white), GCaMP6s (green), and DAPI (blue) are shown. Red arrows point to the OHC stereocilia. C , Mean P2RX2 expression (±SD) in Ocm +/+ and Ocm −/− cochleae at 3–4 weeks with or without noise exposure, normalized to prenoise Ocm +/+ . n = 3 for each genotype under each experimental condition. D , Western blots of P2X2 protein levels in individual cochlea from pre- or postnoise Ocm +/+ and Ocm −/− mice at 3–4 weeks. n = 3 for each genotype under each experiment condition; β-tubulin (loading control) was used for normalization; all values were then normalized to prenoise Ocm +/+ . Significance values are shown as follows: * p < 0.05; ** p < 0.01.

Article Snippet: Samples were labeled with antibodies to myosin 7A (Proteus Biosciences, number 25-6790, 1:200), the C-terminal-binding protein 2 (CtBP2, BD Biosciences #612044, 1:200), OCM (Santa Cruz Biotechnology, sc-7446, 1:200), GluR2 (Millipore, MAB397, 1:200), and P2X2 (Alomone Labs, APR-003, 1:400).

Techniques: Expressing, Immunolabeling, Western Blot, Control, Immunofluorescence, Real-time Polymerase Chain Reaction